Beckman Biomek Fx
Biomek Fx User Manual Software
Biomek Fx User Manual Download

Offset (refer to User’s Manual). Approximately one hour prior to running the automated method, manually turn on the temperature control device and verify that the temperature display on the controller has reached the desired reading. In Biomek software, set both Initialize and End Run Temperature settings at 110 °C. Here is a video showing the Biomek FX framing a position using the Accuframe tool. This takes what was once a tedious and frustrating job of framing your Dec.

High-Definition Biology

No more compromising for quality. With the Biomark™ HD system, you get reliable production-scale throughput and exquisite single-cell sensitivity, with a wide variety of sample types and chemistry choices to accommodate the most genomic applications. Now you can run entire projects from research to validation to routine screening on a single system with the flexibility you want and the consistency you need. Anything else we can help with?

Flexible

Design experiments to match your needs across the widest range of genomic applications.

Sensitive

Detect unique molecular signatures in individual cells and rare cell populations.

Fast

Accelerate discovery with a higher throughput and automated workflow.

Efficient

Conserve precious samples—and funding—with lower reagent use and nanoliter reactions.

Technology

Fluidigm’s revolutionary integrated fluidic circuits (IFCs) empower lifescience research by automating PCR reactions in nanoliter volumes. This meansusing less sample and reagent, and a single microfluidic device, to achieve thehigh-quality, consistent results your work depends on. The Biomark HD systemruns IFCs in either real-time or end-point read modes, bringing flexible,efficient and economical PCR solutions to a range of applications.

Workflow

Prime

Prime the Dynamic Array IFC to prepare it for sample transfer.

Transfer

Pipet samples and primer-probe sets into the designated inlets on the IFC.

Load

Load the IFC on the controller, which prepares the reactions.

Run

Place the IFC into Biomark for thermal cycling and end-point or real-timefluorescence detection.

Analyze

View and analyze color-coded expression heat maps, allele maps and clusterplots for single or multiple runs with our analysis software.

Support

Biomark & EP1 Support

Explore tutorials and publications, browse frequently asked questions, download technical documents, review training programs and service plans and locate the right IFCs, kits and reagents for your research. Still need help? Reach out to one of our support specialists here.

components

IFCs, Assays and Reagents

Digital Array IFCs

Ensure a precise determination of copy number variation with this innovative IFC designed specifically for digital PCR experiments.

Dynamic Array IFCs

Experience a new level of efficiency and throughput for gene expression and genotyping applications with the Fluidigm Dynamic Array™ IFCs used together with the Juno and Biomark HD systems.

SNP Type Assays

SNP Type™ assays provide a high-throughput, low-cost single-nucleotide polymorphism (SNP) genotyping solution enabling rapid assay design for efficient variant screening. The assays are based on allele-specific PCR SNP detection chemistry and are available for use with Fluidigm IFC technology on Biomark HD, EP1™ and Juno™ systems.

D3 Assay Design

Simplify your assay design with our D3™ tool, which enables you to easily define parameters for customized targeted DNA sequencing, gene expression and genotyping. For species not supported online, support is available from the D3 expert design team.

Delta Gene Assays

Realize the full potential of your Biomark™ HD system with minimal setup time and validated protocols that yield quality gene expression results. Just specify your genes of interest and target species to order our exclusive, high-quality, economical and MIQE-compliant Delta Gene™ assays (formerly DELTAgene). Designed specifically for use with Dynamic Array IFCs.

Related Systems

IFC Controllers

Benefit from fewer steps and automated setup of our Dynamic Array™ and Digital Array™ IFCs with Fluidigm’s highly efficient IFC controllers.

Software

Biomark & EP1 Software

The latest software for the Biomark™ HD, Biomark and EP1™ systems. Package includes software for data collection, real-time PCR analysis, genotyping, digital PCR and melting curve analysis. To install, follow the instructions in the Updating the Biomark/EP1 Software Quick Reference (PN 101-6793).

IFC Controllers Software (HX, MX, RX, and WX)

The latest software for IFC Controllers HX, MX, RX and WX. To install, follow the instructions in the Update Software and Scripts section of the relevant IFC Controller User Guide (HX/MX, PN 68000112; RX, PN 100-3385; WX, PN 100-2297).

Singular Analysis Toolset Software

An innovative, open-source solution for analyzing and visualizing data, the Singular™ Analysis Toolsetoffers powerful methods for identifying gene expression and mutation patterns at the single-cell level. Designed for use with Biomark™and C1™ systems.

CopyCount-CNV Software

The cloud-based software analyzes raw fluorescence qPCR data from Biomark™ HD to determine absolute quantification and provide DNA copy number results. Applications include transgene copy number, event-sorting data analysis, and seed zygosity determination.

Each license supports one year of use from the date of activation and may be ordered through your Fluidigm account representative.

GO Immuno-Oncology Workbench

GO Immuno-Oncology Workbench is designed to further analyze primary data obtained with the Advanta™ IO Gene Expression Assay. GO Immuno-Oncology Workbench enables users to analyze large cohorts, discover population-level trends and accelerate checkpoint inhibitor research. It is designed by GenomOncology, a leader in the field of cancer molecular pathology analysis.

Each license supports one year of use from the date of activation and may be ordered through your Fluidigm account representative.

Specifications

Instrument (Part Number BMKHD-BMKHD)

Dimensions
Depth	75 cm (29.5 in)
Width	75 cm (29.5 in)
Height	100 cm (39.4 in)
Weight	140 kg (308 lb)
Thermal Control	Peltier-based, 4–99 ºC
Heating ramp rate	Up to 5.5 ºC/sec
Cooling ramp rate	Up to 5.5 ºC/sec
Detection	CCD camera; 9M pixel, 12 μm x 12 μm pixel size
Fluorescence excitation	475 nm, 530 nm, 575 nm
Fluorescence emission	525 nm, 570 nm, 630 nm
Power requirements	100–230 VAC, 50/60 Hz, 300 W Fluidigm provides a region-specific power cord for the Biomark HD system.
Instrument Control Computer
Computation	Intel® Core™ i3-2120, 3.30 GHz
Memory	2GB
Storage	500 GB HDD
Ports	4 USB 1GB/sec Ethernet
Operating System	Microsoft® Windows® Professiona

Biomek Fx User Manual